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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Simultaneous fluorescence yield and lifetime tomography from time-resolved transmittances of small-animal-sized phantom

Feng Gao, Jiao Li, Limin Zhang, Patrick Poulet, Huijuan Zhao, and Yukio Yamada  »View Author Affiliations


Applied Optics, Vol. 49, Issue 16, pp. 3163-3172 (2010)
http://dx.doi.org/10.1364/AO.49.003163


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Abstract

There has been recently a considerable interest in simultaneously reconstructing yield and lifetime distributions of fluorescent imaging agents inside a bulky tissue, since combined monitoring of these two parameters provides a potential means of in vivo interrogating quantitative and environmental information of specific molecules, as well as accessing interactions among them. It is widely accepted that an advantageous way of accomplishing the task in the context of small-animal imaging is to use a time-domain (TD) modality. In this paper, we present a full three-dimensional, featured-data algorithm for TD diffuse fluorescence tomography, which inverts the Laplace-transformed TD coupled photon diffusion equations and employs a pair of real-domain transform-factors to effectively separate the fluorescent yield and lifetime parameters. By use of a specifically designed 16 × 16 channel time-correlated single photon counting system and a normalized Born formulation for the inversion, the proposed scheme in a transmission mode is experimentally validated to achieve simultaneous reconstruction of the fluorescent yield and lifetime distributions with reasonable accuracy.

© 2010 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 22, 2009
Revised Manuscript: March 15, 2010
Manuscript Accepted: April 30, 2010
Published: May 31, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Feng Gao, Jiao Li, Limin Zhang, Patrick Poulet, Huijuan Zhao, and Yukio Yamada, "Simultaneous fluorescence yield and lifetime tomography from time-resolved transmittances of small-animal-sized phantom," Appl. Opt. 49, 3163-3172 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-16-3163

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