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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Complex refractive index measurement of biological tissues by attenuated total reflection ellipsometry

Jian-Cheng Lai, Ying-Ying Zhang, Zhen-Hua Li, Hai-Jiao Jiang, and An-Zhi He  »View Author Affiliations


Applied Optics, Vol. 49, Issue 16, pp. 3235-3238 (2010)
http://dx.doi.org/10.1364/AO.49.003235


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Abstract

To apply reflection ellipsometry to determine the real and imaginary parts of the refractive index of biological tissues simultaneously, we combine reflection ellipsometry with total internal reflection to warrant minimal influences by the strong scattering and absorption of biological tissues. A K9 glass prism with refractive index 1.51468 at wavelength 632.8 nm and a Glan prism polarizer with an angular sampling interval of 0.1 ° were used in our experimental setup. Using the setup, the complex refractive indices of some typical mammalian tissues were measured under the wavelength of 632.8 nm . The results show that the indices of porcine muscle, liver, pancreas, and dermis tissues were 1.3713 + 0.062 i , 1.3791 + 0.0087 i , 1.3517 + 0.0113 i , and 1.3818 + 0.0049 i , respectively.

© 2010 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: November 30, 2009
Revised Manuscript: March 27, 2010
Manuscript Accepted: April 26, 2010
Published: May 31, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Jian-Cheng Lai, Ying-Ying Zhang, Zhen-Hua Li, Hai-Jiao Jiang, and An-Zhi He, "Complex refractive index measurement of biological tissues by attenuated total reflection ellipsometry," Appl. Opt. 49, 3235-3238 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-16-3235


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References

  1. V. V. Tuchin, “Light scattering study of tissues,” Phys. Usp. 40, 495–515 (1997). [CrossRef]
  2. A. Knuttel and M. B. Godau, “Spatially confined and temporally resolved refractive index and scattering evaluation in human skin performed with optical coherence tomography,” J. Biomed. Opt. 5, 83–92 (2000). [CrossRef] [PubMed]
  3. J. Lai, Z. Li, and A. He, “Influence of complex refractive index on diffuse reflection of biological tissues,” Chin. Phys. Lett. 22, 332–334 (2005). [CrossRef]
  4. A. K. Dunn, “Light scattering properties of cells,” Ph.D. dissertation (University of Texas at Austin, 1997).
  5. F. P. Bolin, L. E. Preuss, and R. C. Talyor, “Refractive index of some mammalian tissues using a fiber optic cladding method,” Appl. Opt. 28, 2297–2303 (1989). [CrossRef] [PubMed]
  6. G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, “Determination of the refractive index of high scattering human tissue by optical coherence tomography,” Opt. Lett. 20, 2258–2260 (1995). [CrossRef] [PubMed]
  7. S. A. Alexandrov, A. V. Zvyagin, K. K. M. B. Dilusha Silva, and D. D. Sampson, “Bifocal optical coherence refractometry of turbid media,” Opt. Lett. 28, 117–119 (2003). [CrossRef] [PubMed]
  8. J. J. J. Dirckx, L. C. Kuypers, and W. F. Decraemer, “Refractive index of tissue measured with confocal microscopy,” J. Biomed. Opt. 10, 044014 (2005). [CrossRef]
  9. V. Tsenova and E. Stoykova, “Refractive index measurement in human tissue samples,” Proc. SPIE 5226, 413–417 (2003). [CrossRef]
  10. H. Li and S. Xie, “Measurement method of the refractive index of biotissue by total internal reflection,” Appl. Opt. 35, 1793–1795 (1996). [CrossRef] [PubMed]
  11. J. Lai, Z. Li, C. Wang, and A. He, “Experimental measurement of the refractive index of biological tissues by total internal reflection,” Appl. Opt. 44, 1845–1849 (2005). [CrossRef] [PubMed]
  12. H. Ding, J. Q. Lu, K. M. Jacobs, and X. Hu, “Determination of refractive indices of porcine skin tissues and Intralipid at eight wavelengths between 325 and 1557nm,” J. Opt. Soc. Am. A 22, 1151–1157 (2005). [CrossRef]
  13. H. Ding, J. Q. Lu, W. A. Wooden, P. J. Kragel, and X. H. Hu, “Refractive indices of human skin tissues at eight wavelengths and estimated dispersion relations between 300 and 1600nm,” Phys. Med. Biol. 51, 1479–1489 (2006). [CrossRef] [PubMed]
  14. E. Hecht, Optics (Addison-Wesley, 2002), p. 66.

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