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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 12 — Sep. 30, 2010

Microscope spectrometer for light scattering investigations

Aude Barbara, Tomas Lopez-Rios, Sylvain Dumont, Frédéric Gay, and Pascal Quémerais  »View Author Affiliations

Applied Optics, Vol. 49, Issue 22, pp. 4193-4201 (2010)

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We describe a setup including a microscope to study volumes of a few μm 3 by static and dynamic light scattering (DLS) in a backscattering configuration. Light scattered by individual objects of micrometric size can be analyzed in the 400 800 nm spectral range. This setup can also be employed to study both diluted and concentrated colloidal solutions by DLS measurements. For diluted solutions we found evidence of the fluctuations of the number of particles in a confocal volume. We discuss their contribution to the autocorrelation function of the scattered intensity measured as a function of time.

© 2010 Optical Society of America

OCIS Codes
(120.4820) Instrumentation, measurement, and metrology : Optical systems
(290.5820) Scattering : Scattering measurements
(120.6165) Instrumentation, measurement, and metrology : Speckle interferometry, metrology

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: April 12, 2010
Revised Manuscript: June 3, 2010
Manuscript Accepted: June 28, 2010
Published: July 27, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Aude Barbara, Tomas Lopez-Rios, Sylvain Dumont, Frédéric Gay, and Pascal Quémerais, "Microscope spectrometer for light scattering investigations," Appl. Opt. 49, 4193-4201 (2010)

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