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Ultraviolet analysis of donated corneas: a portable prototype

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Abstract

As technology improves human vision, some procedures currently performed may be causing a decrease of the natural UV protection of the cornea. A portable dual beam system prototype was assembled for physicians for clinical studies of these effects on the corneas endowing two types of 300–400 nm evaluations: 1, regularly donated corneas and 2, simulating refractive keratectomy by corneal lamellae removal. The system performs 500 measurements/s, providing ±0.25% precision for the transmittance. The measurements performed on the prototype are 95% in agreement with Cary 17 and HR4000CG-UV-NIR Ocean Optics spectrophotometers. Preliminary studies on cadaveric corneas demonstrate that, as the stromal layer is reduced (150μm depth), there is significant loss—an average of 7.1%.—of the cornea’s natural UV protection. The prototype is being tested in an eye bank for routine evaluation of donor corneas.

© 2010 Optical Society of America

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