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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Experimental and theoretical evaluation of a fiber-optic approach for optical property measurement in layered epithelial tissue

Quanzeng Wang, Karthik Shastri, and T. Joshua Pfefer  »View Author Affiliations


Applied Optics, Vol. 49, Issue 28, pp. 5309-5320 (2010)
http://dx.doi.org/10.1364/AO.49.005309


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Abstract

Improvements in measurement of epithelial tissue optical properties (OPs) in the ultraviolet and visible (UV–Vis) may lead to enhanced understanding of optical techniques for neoplasia detection. In this study, we investigated an approach based on fiber-optic measurement of reflectance to determine absorption and reduced scattering coefficients ( μ a and μ s ) in two-layer turbid media. Neural network inverse models were trained on simulation data for a wide variety of OP combinations ( μ a = 1 22.5 , μ s = 5 42.5 cm 1 ). Experimental measurements of phantoms with top-layer thicknesses (D) ranging from 0.22 to 0.66 mm were performed at three UV–Vis wavelengths. OP estimation accuracy was calculated and compared to theoretical results. Mean prediction errors were strongly correlated with D and ranged widely, from 1.5 to 12.1 cm 1 . Theoretical analyses indicated the potential for improving accuracy with alternate probe geometries. Although numerous challenges remain, this initial experimental study of an unconstrained approach for fiber-optic-based OP determination in two-layer epithelial tissue indicates the potential to provide useful measurements.

© 2010 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.4630) Instrumentation, measurement, and metrology : Optical inspection
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.1030) Spectroscopy : Absorption
(300.6540) Spectroscopy : Spectroscopy, ultraviolet

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 14, 2010
Revised Manuscript: August 17, 2010
Manuscript Accepted: August 22, 2010
Published: September 24, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Citation
Quanzeng Wang, Karthik Shastri, and T. Joshua Pfefer, "Experimental and theoretical evaluation of a fiber-optic approach for optical property measurement in layered epithelial tissue," Appl. Opt. 49, 5309-5320 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-28-5309


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