OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns

Steffen Bjørn Petersen, Ane Kold di Gennaro, Maria Teresa Neves-Petersen, Esben Skovsen, and Antonietta Parracino  »View Author Affiliations


Applied Optics, Vol. 49, Issue 28, pp. 5344-5350 (2010)
http://dx.doi.org/10.1364/AO.49.005344


View Full Text Article

Enhanced HTML    Acrobat PDF (833 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer ( 0.7 μm ) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed.

© 2010 Optical Society of America

OCIS Codes
(310.1860) Thin films : Deposition and fabrication
(350.6670) Other areas of optics : Surface photochemistry
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Fourier Optics and Signal Processing

History
Original Manuscript: July 8, 2010
Manuscript Accepted: July 15, 2010
Published: September 24, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Citation
Steffen Bjørn Petersen, Ane Kold di Gennaro, Maria Teresa Neves-Petersen, Esben Skovsen, and Antonietta Parracino, "Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns," Appl. Opt. 49, 5344-5350 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-28-5344

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited