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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns

Steffen Bjørn Petersen, Ane Kold di Gennaro, Maria Teresa Neves-Petersen, Esben Skovsen, and Antonietta Parracino  »View Author Affiliations

Applied Optics, Vol. 49, Issue 28, pp. 5344-5350 (2010)

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We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer ( 0.7 μm ) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed.

© 2010 Optical Society of America

OCIS Codes
(310.1860) Thin films : Deposition and fabrication
(350.6670) Other areas of optics : Surface photochemistry
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Fourier Optics and Signal Processing

Original Manuscript: July 8, 2010
Manuscript Accepted: July 15, 2010
Published: September 24, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Steffen Bjørn Petersen, Ane Kold di Gennaro, Maria Teresa Neves-Petersen, Esben Skovsen, and Antonietta Parracino, "Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns," Appl. Opt. 49, 5344-5350 (2010)

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