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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 1 — Jan. 3, 2011

Depth-resolved velocimetry of Hagen–Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy

Michael Esseling, Frank Holtmann, Mike Woerdemann, and Cornelia Denz  »View Author Affiliations

Applied Optics, Vol. 49, Issue 31, pp. 6030-6038 (2010)

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We quantitatively investigate the axial imaging properties of dynamic phase-contrast microscopy, with a special focus on typical combinations of tracer particles and magnifications that are used for velocimetry analysis. We show, for the first time, that a dynamic phase-contrast microscope, which is the integration of an all-optical novelty filter in a commercially available inverted microscope, can visualize three- dimensional velocity fields with a significantly reduced optical sectioning depth. The depth of field for dynamic phase-contrast microscopy is extracted from the three-dimensional response function and compared with the respective values for incoherent bright-field illumination. These results are then used to perform a depth-resolved particle image velocimetry analysis of Hagen–Poiseuille as well as electro-osmotically actuated flows in a microchannel.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.7070) Nonlinear optics : Two-wave mixing
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: July 30, 2010
Manuscript Accepted: September 14, 2010
Published: October 26, 2010

Virtual Issues
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Michael Esseling, Frank Holtmann, Mike Woerdemann, and Cornelia Denz, "Depth-resolved velocimetry of Hagen–Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy," Appl. Opt. 49, 6030-6038 (2010)

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