We quantitatively investigate the axial imaging properties of dynamic phase-contrast microscopy, with a special focus on typical combinations of tracer particles and magnifications that are used for velocimetry analysis. We show, for the first time, that a dynamic phase-contrast microscope, which is the integration of an all-optical novelty filter in a commercially available inverted microscope, can visualize three- dimensional velocity fields with a significantly reduced optical sectioning depth. The depth of field for dynamic phase-contrast microscopy is extracted from the three-dimensional response function and compared with the respective values for incoherent bright-field illumination. These results are then used to perform a depth-resolved particle image velocimetry analysis of Hagen–Poiseuille as well as electro-osmotically actuated flows in a microchannel.
© 2010 Optical Society of America
Original Manuscript: July 30, 2010
Manuscript Accepted: September 14, 2010
Published: October 26, 2010
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics
Michael Esseling, Frank Holtmann, Mike Woerdemann, and Cornelia Denz, "Depth-resolved velocimetry of Hagen–Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy," Appl. Opt. 49, 6030-6038 (2010)