Spectral domain fluorescence coherence phase microscopy

Andra St. Quintin; Lukas-Karim Merhi; Marinko V. Sarunic

doi:10.1364/AO.50.001798

Applied Optics
Vol. 50,
Issue 12,
pp. 1798-1804
(2011)
•https://doi.org/10.1364/AO.50.001798

Spectral domain fluorescence coherence phase microscopy

Andra St. Quintin, Lukas-Karim Merhi, and Marinko V. Sarunic

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Andra St. Quintin, Lukas-Karim Merhi, and Marinko V. Sarunic, "Spectral domain fluorescence coherence phase microscopy," Appl. Opt. 50, 1798-1804 (2011)

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- Microscopy
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About this Article
History
- Original Manuscript: January 13, 2011
- Manuscript Accepted: February 7, 2011
- Published: April 19, 2011
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Virtual Journal for Biomedical Optics Vol. 6, Iss. 5

Abstract

Spectral domain phase microscopy (SDPM) has been reported in the literature as a functional extension to low-coherence interferometry, which enables nanoscale measurement of a scatter’s displacement. The signal in SDPM is generated from structural images that lack molecular specificity. This paper investigates the expansion of phase analysis to fluorescence self-interference signals to provide functional information about a sample. Spectral domain fluorescence coherence phase microscopy is demonstrated for nanoscale resolution motion detection of fluorescent particles with a signal-to-noise ratio limited resolution of $~ 10 nm$ . This paper demonstrates the feasibility of combining phase processing with fluorescence self-interference, which may be useful for future applications such as cell rheology.

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Parameter	α	β	γ	ε	ζ
$a (rad)$	$0.064 \pm 0.008$	$0.129 \pm 0.009$	$0.203 \pm 0.006$	$0.302 \pm 0.008$	$0.372 \pm 0.009$
$b (Hz)$	$0.509 \pm 0.007$	$0.511 \pm 0.003$	$0.511 \pm 0.002$	$0.512 \pm 0.001$	$0.511 \pm 0.001$
c (unitless)	$2.62 \pm 0.3$	$- 2.23 \pm 0.1$	$- 1.55 \pm 0.06$	$1.54 \pm 0.05$	$- 2.38 \pm 0.05$
$d (rad / s)$	$- 0.004 \pm 0.002$	$0.004 \pm 0.002$	$0.003 \pm 0.002$	$0.007 \pm 0.002$	$0.0004 \pm 0.003$
$e (rad)$	$1.08 \pm 0.01$	$2.20 \pm 0.01$	$3.479 \pm 0.009$	$4.96 \pm 0.01$	$6.74 \pm 0.01$

Case	Peak-to-Peak Motion (nm)	Expected Shot-Noise Limit (nm)
α	$6.1 \pm 0.7$	8.59
β	$12.3 \pm 0.8$	9.52
γ	$19.4 \pm 0.6$	7.99
ε	$28.8 \pm 0.7$	7.43
ζ	$35.5 \pm 0.8$	8.81

Parameter	α	β	γ	ε	ζ
$a (rad)$	$0.064 \pm 0.008$	$0.129 \pm 0.009$	$0.203 \pm 0.006$	$0.302 \pm 0.008$	$0.372 \pm 0.009$
$b (Hz)$	$0.509 \pm 0.007$	$0.511 \pm 0.003$	$0.511 \pm 0.002$	$0.512 \pm 0.001$	$0.511 \pm 0.001$
c (unitless)	$2.62 \pm 0.3$	$- 2.23 \pm 0.1$	$- 1.55 \pm 0.06$	$1.54 \pm 0.05$	$- 2.38 \pm 0.05$
$d (rad / s)$	$- 0.004 \pm 0.002$	$0.004 \pm 0.002$	$0.003 \pm 0.002$	$0.007 \pm 0.002$	$0.0004 \pm 0.003$
$e (rad)$	$1.08 \pm 0.01$	$2.20 \pm 0.01$	$3.479 \pm 0.009$	$4.96 \pm 0.01$	$6.74 \pm 0.01$

Case	Peak-to-Peak Motion (nm)	Expected Shot-Noise Limit (nm)
α	$6.1 \pm 0.7$	8.59
β	$12.3 \pm 0.8$	9.52
γ	$19.4 \pm 0.6$	7.99
ε	$28.8 \pm 0.7$	7.43
ζ	$35.5 \pm 0.8$	8.81

Abstract

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Equations (3)

Applied Optics