OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 8 — Aug. 26, 2011

Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy

Tze-Hsuan Liu, Jian-Long Xiao, Chau-Hwang Lee, and Jiunn-Yuan Lin  »View Author Affiliations


Applied Optics, Vol. 50, Issue 19, pp. 3311-3315 (2011)
http://dx.doi.org/10.1364/AO.50.003311


View Full Text Article

Enhanced HTML    Acrobat PDF (451 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We use optical tweezers to trap a unilamellar phospholipid vesicle and measure the out-of-plane thermal fluctuations by using differential confocal microscopy. Bending moduli of the lipid membranes are calculated directly from the mean-square values of the fluctuation amplitudes. Owing to the refractive index contrast between the inner and outer solutions of the vesicle, optical tweezers trap the vesicle laterally and improve the reliability of the measured fluctuation amplitudes along the optical axis. Bending moduli of membranes in gel or fluid phases obtained by the combination of differential confocal microscopy and optical tweezers are close to those reported previously. We also obtain the bending modulus of sphingomyelin membranes in the gel phase, which was not reported previously.

© 2011 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1790) Medical optics and biotechnology : Confocal microscopy

ToC Category:
Lasers and Laser Optics

History
Original Manuscript: April 14, 2011
Revised Manuscript: May 15, 2011
Manuscript Accepted: May 16, 2011
Published: July 1, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Tze-Hsuan Liu, Jian-Long Xiao, Chau-Hwang Lee, and Jiunn-Yuan Lin, "Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy," Appl. Opt. 50, 3311-3315 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-50-19-3311


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. Dimova, S. Aranda, N. Bezlyepkina, V. Nikolov, K. A. Riske, and R. Lipowsky, “Practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy,” J. Phys. Condens. Matter 18, S1151–S1176 (2006). [CrossRef] [PubMed]
  2. G. Tresset and S. Takeuchi, “Utilization of cell-sized lipid containers for nanostructure and macromolecule handling in microfabricated devices,” Anal. Chem. 77, 2795–2801(2005). [CrossRef] [PubMed]
  3. A. P. Liu, D. L. Richmond, L. Maibaum, S. Pronk, P. L. Geissler, and D. A. Fletcher, “Membrane-induced bundling of actin filaments,” Nat. Phys. 4, 789–793 (2008). [CrossRef]
  4. C. B. Fox, R. A. Horton, and J. M. Harris, “Detection of drug-membrane interactions in individual phospholipid vesicles by confocal Raman microscopy,” Anal. Chem. 78, 4918–4924(2006). [CrossRef] [PubMed]
  5. R. Lipowsky, “Budding of membranes induced by intramembrane domains,” J. Phys. II 2, 1825–1840 (1992). [CrossRef]
  6. E. Evans and W. Rawicz, “Entropy-driven tension and bending elasticity in condensed-fluid membranes,” Phys. Rev. Lett. 64, 2094–2097 (1990). [CrossRef] [PubMed]
  7. H. P. Duwe, J. Kaes, and E. Sackmann, “Bending elastic moduli of lipid bilayers-modulation by solutes,” J. Phys. 51, 945–962 (1990). [CrossRef]
  8. M. D. Mitov, J. F. Faucon, P. Meleard, and P. Bothorel, “Thermal fluctuations of membranes,” in Advances in Supramolecular Chemistry, G.W.Gokel, ed. (JAI Press, 1992), Vol.  2, pp. 93–139.
  9. P. M. Chaikin and T. C. Lubensky, Principles of Condensed Matter Physics (Cambridge University, 1995), Section 10.4.
  10. C.-H. Lee and J. Wang, “Noninterferometric differential confocal microscopy with 2 nm depth resolution,” Opt. Commun. 135, 233–237 (1997). [CrossRef]
  11. T. Heimburg, “Mechanical aspects of membrane thermodynamics. Estimation of the mechanical properties of lipid membranes close to the chain melting transition from calorimetry,” Biochim. Biophys. Acta 1415, 147–162 (1998). [CrossRef] [PubMed]
  12. M. I. Angelova, S. Soleau, P. Meleard, J. F. Faucon, and P. Bothorel, “Preparation of giant vesicles by external AC electric fields: kinetics and applications,” Prog. Colloid Polym. Sci. 89, 127–131 (1992). [CrossRef]
  13. C.-H. Lee, Y.-F. Chang, C.-H. Tsai, and P.-H. Wang, “Optical measurement of the deformation of giant lipid vesicles driven by a micropipet electrode,” Langmuir 21, 7186–7190 (2005). [CrossRef] [PubMed]
  14. A. Ashkin, “Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime,” Biophys. J. 61, 569–582 (1992). [CrossRef] [PubMed]
  15. J. Henriksen, A. C. Rowat, and J. H. Ipsen, “Vesicle fluctuation analysis of the effects of sterols on membrane bending rigidity,” Eur. Biophys. J. 33, 732–741 (2004). [CrossRef] [PubMed]
  16. C.-H. Lee, W.-C. Lin, and J. Wang, “All-optical measurements of the bending rigidity of lipid-vesicle membranes across structural phase transitions,” Phys. Rev. E 64, 020901 (2001). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited