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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 8 — Aug. 26, 2011

Dual-order snapshot spectral imaging of plasmonic nanoparticles

Gregory J. Nusz, Stella M. Marinakos, Srinath Rangarajan, and Ashutosh Chilkoti  »View Author Affiliations

Applied Optics, Vol. 50, Issue 21, pp. 4198-4206 (2011)

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The development of truly scalable, multiplexed optical microarrays requires a detection platform capable of simultaneous detection of multiple signals in real-time. We present a technique we term dual-order snapshot spectroscopic imaging (DOSSI) and demonstrate that it can be effectively used to collect spectrally resolved images of a full field of view of sparsely located spots in real time. Resonant peaks of plasmonic gold nanoparticles were tracked as a function of their surrounding refractive index. Measurement uncertainty analysis indicated that the spectral resolution of DOSSI in the described configuration is approximately 0.95 nm . Further, real-time measurements by DOSSI allowed discrimination between optically identical nanoparticles that were functionalized with two homologous small molecule ligands that bound to the same protein, albeit with different affinity, based purely on their different molecular interaction kinetics—a feat not possible with slower raster-type hyperspectral imaging systems, or other dark-field optical detection systems that solely rely on end point measurements. Kinetic measurements of plasmon bands by DOSSI can be performed with a relatively simple optical system, thereby opening up the possibility of developing low-cost detectors for arrayed plasmonic diagnostics.

© 2011 Optical Society of America

OCIS Codes
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Imaging Systems

Original Manuscript: February 23, 2011
Revised Manuscript: June 6, 2011
Manuscript Accepted: June 9, 2011
Published: July 19, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Gregory J. Nusz, Stella M. Marinakos, Srinath Rangarajan, and Ashutosh Chilkoti, "Dual-order snapshot spectral imaging of plasmonic nanoparticles," Appl. Opt. 50, 4198-4206 (2011)

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