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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 3 — Mar. 18, 2011

Effect of growth media and washing on the spectral signatures of aerosolized biological simulants

Christian Laflamme, Jean-Robert Simard, Sylvie Buteau, Pierre Lahaie, Denis Nadeau, Bernard Déry, Olivier Houle, Pierre Mathieu, Gilles Roy, Jim Ho, and Caroline Duchaine  »View Author Affiliations


Applied Optics, Vol. 50, Issue 6, pp. 788-796 (2011)
http://dx.doi.org/10.1364/AO.50.000788


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Abstract

We have evaluated the influence of growth media and washing on the laser-induced fluorescence spectra of bacteria. Three different bacterial simulants were cultured in three types of growth media. Three kinds of samples were generated from each culture: the culture itself, the growth medium alone, and a triple-washed sample. The materials were injected as aerosols in a lab-sized lidar aerosol chamber to obtain their spectra. Using two different analysis approaches, signature variations were observed between the three kinds of samples for most combinations of growth media/bacteria. This study concludes that the culture media used influences the spectral signatures.

OCIS Codes
(260.7190) Physical optics : Ultraviolet
(280.1100) Remote sensing and sensors : Aerosol detection
(280.3640) Remote sensing and sensors : Lidar
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6170) Spectroscopy : Spectra
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Remote Sensing and Sensors

History
Original Manuscript: October 7, 2010
Manuscript Accepted: November 18, 2010
Published: February 10, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Christian Laflamme, Jean-Robert Simard, Sylvie Buteau, Pierre Lahaie, Denis Nadeau, Bernard Déry, Olivier Houle, Pierre Mathieu, Gilles Roy, Jim Ho, and Caroline Duchaine, "Effect of growth media and washing on the spectral signatures of aerosolized biological simulants," Appl. Opt. 50, 788-796 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-50-6-788

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