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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Optical sectioning with a low-coherence phase-shifting digital holographic microscope

Yu-Chih Lin, Chau-Jern Cheng, and Ting-Chung Poon  »View Author Affiliations

Applied Optics, Vol. 50, Issue 7, pp. B25-B30 (2011)

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The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3 μm and axial resolution of 10 μm through a thickness of 300 μm onion membrane. In addition, with the above said resolu tions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic micro scope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.

© 2011 Optical Society of America

OCIS Codes
(110.4980) Imaging systems : Partial coherence in imaging
(090.1995) Holography : Digital holography

Original Manuscript: July 28, 2010
Revised Manuscript: October 28, 2010
Manuscript Accepted: October 31, 2010
Published: December 6, 2010

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Yu-Chih Lin, Chau-Jern Cheng, and Ting-Chung Poon, "Optical sectioning with a low-coherence phase-shifting digital holographic microscope," Appl. Opt. 50, B25-B30 (2011)

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