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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Numerical evaluation of droplet sizing based on the ratio of fluorescent and scattered light intensities (LIF/Mie technique)

Georgios Charalampous and Yannis Hardalupas  »View Author Affiliations


Applied Optics, Vol. 50, Issue 9, pp. 1197-1209 (2011)
http://dx.doi.org/10.1364/AO.50.001197


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Abstract

The dependence of fluorescent and scattered light intensities from spherical droplets on droplet diameter was evaluated using Mie theory. The emphasis is on the evaluation of droplet sizing, based on the ratio of laser-induced fluorescence and scattered light intensities (LIF/Mie technique). A parametric study is presented, which includes the effects of scattering angle, the real part of the refractive index and the dye concentration in the liquid (determining the imaginary part of the refractive index). The assumption that the fluorescent and scattered light intensities are proportional to the volume and surface area of the droplets for accurate sizing measurements is not generally valid. More accurate sizing measurements can be performed with minimal dye concentration in the liquid and by collecting light at a scattering angle of 60 ° rather than the commonly used angle of 90 ° . Unfavorable to the sizing accuracy are oscillations of the scattered light intensity with droplet diameter that are profound at the sidescatter direction ( 90 ° ) and for droplets with refractive indices around 1.4.

© 2011 Optical Society of America

OCIS Codes
(120.3940) Instrumentation, measurement, and metrology : Metrology
(280.1100) Remote sensing and sensors : Aerosol detection
(290.5850) Scattering : Scattering, particles
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Scattering

History
Original Manuscript: October 27, 2010
Revised Manuscript: January 11, 2011
Manuscript Accepted: January 14, 2011
Published: March 10, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Georgios Charalampous and Yannis Hardalupas, "Numerical evaluation of droplet sizing based on the ratio of fluorescent and scattered light intensities (LIF/Mie technique)," Appl. Opt. 50, 1197-1209 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-50-9-1197

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