OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy

M. H. Jericho, H. J. Kreuzer, M. Kanka, and R. Riesenberg  »View Author Affiliations


Applied Optics, Vol. 51, Issue 10, pp. 1503-1515 (2012)
http://dx.doi.org/10.1364/AO.51.001503


View Full Text Article

Enhanced HTML    Acrobat PDF (1254 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Point-source digital in-line holographic microscopy with numerical reconstruction is ideally suited for quantitative phase measurements to determine optical path lengths and to extract changes in refractive index within accuracy close to 0.001 on the submicrometer length scale. This is demonstrated with simulated holograms and with detailed measurements on a number of different micrometer-sized samples such as suspended drops, optical fibers, as well as organisms of biological interest such as E. coli bacteria, HeLa cells, and fibroblast cells.

© 2012 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(110.0110) Imaging systems : Imaging systems
(110.2960) Imaging systems : Image analysis
(090.1995) Holography : Digital holography
(110.5086) Imaging systems : Phase unwrapping

ToC Category:
Imaging Systems

History
Original Manuscript: September 23, 2011
Revised Manuscript: November 14, 2011
Manuscript Accepted: November 15, 2011
Published: March 28, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
M. H. Jericho, H. J. Kreuzer, M. Kanka, and R. Riesenberg, "Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy," Appl. Opt. 51, 1503-1515 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-10-1503


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, “Digital in-line holography for biological applications,” Proc. Natl. Acad. Sci. USA 98, 11301–11305 (2001). [CrossRef]
  2. J. Garcia-Sucerquia, W. Xu, S. K. Jericho, M. H. Jericho, P. Klages, and H. J. Kreuzer, “Digital in-line holographic microscopy,” Appl. Opt. 45, 836–850 (2006). [CrossRef]
  3. H. J. Kreuzer, M. H. Jericho, I. A. Meinertzhagen, and W. B. Xu, “Digital in-line holography with photons and electrons,” J. Phys. Condens. Matter 13, 10729–10741 (2001). [CrossRef]
  4. M. Kanka, R. Riesenberg, and H. J. Kreuzer, “Reconstruction of high-resolution holographic microscopic images,” Opt. Lett. 34, 1162–1164 (2009). [CrossRef]
  5. H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, “Theory of the point source electron microscope,” Ultramicroscopy 45, 381–403 (1992). [CrossRef]
  6. M. H. Jericho and H. J. Kreuzer, “Point source digital in-line holographic microscopy,” in Coherent Light Microscopy, Vol. 46 of Springer Series in Surface Sciences, P. Ferraro, A. Wax, and Z. Zalevsky, eds. (Springer-Verlag, 2011), pp. 3–30. [CrossRef]
  7. W. Qu, O. C. Chee, Y. Yu, and A. Asundi, “Recording and reconstruction of digital Gabor holograms,” Optik 121, 2179–2184 (2010). [CrossRef]
  8. D. Gabor, “Microscopy by reconstructed wave fronts,” Proc. R. Soc. A 197, 454–487 (1949). [CrossRef]
  9. S. K. Jericho, J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, “Submersible digital in-line holographic microscope,” Rev. Sci. Instrum. 77, 043706 (2006). [CrossRef]
  10. www.resolutionoptics.com .
  11. A. Wuttig, M. Kanka, H. J. Kreuzer, and R. Ranier, “Packed domain Rayleigh–Sommerfeld wavefield propagation for large targets,” Opt. Express 18, 27036–27047 (2010). [CrossRef]
  12. J. Y. Lee, C. W. Lee, E. H. Lin, and P. K. Wei, “Single live cell refractometer using nanoparticle coated fiber tip,” Appl. Phys. Lett. 93, 173110 (2008). [CrossRef]
  13. F. Lanni, A. S. Waggoner, and D. L. Taylor, “Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy,” J. Cell Biol. 100, 1091–1102 (1985). [CrossRef]
  14. N. Lue, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, ”Live cell refractometry using microfluidic devices,” Opt. Lett. 31, 2759–2761 (2006). [CrossRef]
  15. A. P. Kononenko, K. I. Kononenko, and D. M. Mikhov, “Dependence of refractive index on physiological state of microbial population,” Zh. Prikl. Spektrosk. 11, 114–117 (1969), translation.
  16. S. P. Anokhov, “Plane wave diffraction by a perfectly transparent half-plane,” J. Opt. Soc. Am. 24, 2493–2498 (2007). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited