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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

Ion concentration quantification with the help of single-wavelength dye fluorescence

Y. Zakharov, A. Ershova, N. Golubkin, and I. Mukhina  »View Author Affiliations


Applied Optics, Vol. 51, Issue 10, pp. C95-C99 (2012)
http://dx.doi.org/10.1364/AO.51.000C95


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Abstract

This paper discusses the task of true physical quantity reconstruction from experimental data on the basis of fluorescence microscopy images when a specific ion indicator is used for an investigation into ion concentration. Brain cell functional activity in the form of calcium oscillation may be processed as quantitation on the basis of measurements of single-wavelength fluorescence intensity using data about dye loading protocol, excitation, and detection conditions without calibration of every experiment.

© 2012 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

History
Original Manuscript: December 7, 2011
Revised Manuscript: February 9, 2012
Manuscript Accepted: February 10, 2012
Published: March 28, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Y. Zakharov, A. Ershova, N. Golubkin, and I. Mukhina, "Ion concentration quantification with the help of single-wavelength dye fluorescence," Appl. Opt. 51, C95-C99 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-10-C95


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References

  1. J. B. Pawley, ed., Handbook of Biological Confocal Microscopy (Springer, 2006).
  2. M. Zaidi, A. S. M Towhidul Alam, Ch. Bax, V. Shankar, P. F. R. Bevis, Ch. L. H. Huang, M. Pazianas, and B. S. Moonga, “Cytosolic free calcium measurements in single cell using calcium-sensitive fluorochromes,” in Methods in Molecular Biology, J. Graham and J. A. Higgins, eds. (Humana, 1994), pp. 279–293.
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  5. I. V. Mukhina, V. B. Kazantsev, L. G. Khaspekov, Yu. N. Zackharov, M. V. Vedunova, E. V. Mitroshina, S. A. Korotchenko, and E. A. Koryagina, “Multielectrode arrays—new possibilities in investigation of the neuronal network plasticity,” Mod. Tech. Med.1, 8–15 (2009).
  6. BioProbes, “Fluorescent calcium indicators for in vivoimaging of neuronal networks” (Invitrogen, 2007), Vol. 54, pp. 26–27.
  7. S. Namiki, T. Sasaki, N. Matsuski, and Y. Ikegaya, “Regional difference in stainability with calcium-sensitive acetoxymetyl-ester probes in mice brain slices,” Int. J. Neurosci. 119, 214–216 (2008).

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