|
Ion concentration quantification with the help of single-wavelength dye fluorescence |
Applied Optics, Vol. 51, Issue 10, pp. C95-C99 (2012)
http://dx.doi.org/10.1364/AO.51.000C95
Enhanced HTML 
Acrobat PDF (641 KB)
Abstract
This paper discusses the task of true physical quantity reconstruction from experimental data on the basis of fluorescence microscopy images when a specific ion indicator is used for an investigation into ion concentration. Brain cell functional activity in the form of calcium oscillation may be processed as quantitation on the basis of measurements of single-wavelength fluorescence intensity using data about dye loading protocol, excitation, and detection conditions without calibration of every experiment.
© 2012 Optical Society of America
OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
History
Original Manuscript: December 7, 2011
Revised Manuscript: February 9, 2012
Manuscript Accepted: February 10, 2012
Published: March 28, 2012
Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics
Citation
Y. Zakharov, A. Ershova, N. Golubkin, and I. Mukhina, "Ion concentration quantification with the help of single-wavelength dye fluorescence," Appl. Opt. 51, C95-C99 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-10-C95
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Microscopic fluorescence imaging of bulk defect clusters in KH2PO4 crystals (OL)
- Photostability of a Fluorescent Marker Under Pulsed Excited-State Depletion through Stimulated Emission (AO)
- Single-molecule orientations determined by direct emission pattern imaging (JOSAB)
- Increasing the lateral resolution of 4Pi fluorescence microscopes (JOSAA)
- Absolute measurement of molecular two-photon absorption cross-sections using a fluorescence saturation technique (OE)
Related Conference Papers
- Experimental and theoretical investigations of environmentally sensitive single-molecule fluorophores
- Optically sensing the state of a single molecule
- Multidimensional fluorescence spectroscopy of single molecules
- Imaging Single Molecule Spectroscopy in Densely Labelled Samples
- Optical Manipulation of Femtoliter Aqueous Droplets for Nanochemistry Applications
« Previous Article | Next Article »
OSA is a member of 