We describe a multispectral continuous-wave diffuse optical tomography (DOT) system that can be used for in vivo three-dimensional (3-D) imaging of seizure dynamics. Fast 3-D data acquisition is realized through a time multiplexing approach based on a parallel lighting configuration—our system can achieve 0.12 ms per source per wavelength and up to a 14 Hz sampling rate for a full set of data for 3-D DOT image reconstruction. The system is validated using both static and dynamic tissue-like phantoms. An initial in vivo experiment using a rat model of seizure is also demonstrated.
© 2012 Optical Society of America
Original Manuscript: January 13, 2012
Revised Manuscript: March 9, 2012
Manuscript Accepted: March 9, 2012
Published: May 31, 2012
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics
Jianjun Yang, Tao Zhang, Hao Yang, and Huabei Jiang, "Fast multispectral diffuse optical tomography system for in vivo three-dimensional imaging of seizure dynamics," Appl. Opt. 51, 3461-3469 (2012)