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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Imaging of oocyte development using ultrahigh-resolution full-field optical coherence tomography

Jiaying Xiao, Bo Wang, Guangyin Lu, Ziqiang Zhu, and Yujiao Huang  »View Author Affiliations

Applied Optics, Vol. 51, Issue 16, pp. 3650-3654 (2012)

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In this paper, a white-light full-field optical coherence tomography is developed to provide three-dimensional imaging of the development of a mouse embryo with ultrahigh-resolution. Spatial resolution of 1.8μm×1.12μm (transverse×axial) is achieved owing to the extremely short coherence length of the light source and optimized compensation of dispersion mismatch. A shot-noise limited detection sensitivity of 80 dB is obtained at an acquisition time of 5 seconds per image. To enable in vivo imaging of the mouse embryo development, a homemade incubator is applied to provide appropriate CO2 concentration, temperature, and humidity. An electronic light shutter is used to control the light source in order to avoid unnecessary exposure to the embryo development when the sample is not being scanned. To demonstrate our method, in vivo time series two-dimensional images of the in vitro fertilization process of mouse oocytes at the germinal vesicles stage are presented.

© 2012 Optical Society of America

ToC Category:
Imaging Systems

Original Manuscript: November 15, 2011
Manuscript Accepted: December 6, 2011
Published: June 1, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Jiaying Xiao, Bo Wang, Guangyin Lu, Ziqiang Zhu, and Yujiao Huang, "Imaging of oocyte development using ultrahigh-resolution full-field optical coherence tomography," Appl. Opt. 51, 3650-3654 (2012)

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