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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 10 — Oct. 5, 2012

Optimization of probe-laser focal offsets for single-particle tracking

Ai-Tang Chang, Yi-Ren Chang, Sien Chi, and Long Hsu  »View Author Affiliations


Applied Optics, Vol. 51, Issue 23, pp. 5643-5648 (2012)
http://dx.doi.org/10.1364/AO.51.005643


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Abstract

In optical tweezers applications, tracking a trapped particle is essential for force measurement. One of the most popular techniques for single-particle tracking is achieved by analyzing the forward and backward light pattern, scattered by the target particle trapped by a trap laser beam, of an additional probe-laser beam with different wavelength whose focus is slightly apart from the trapping center. However, the optimized focal offset has never been discussed. In this paper, we investigate the tracking range and sensitivity as a function of the focal offset between the trapping and the probe-laser beams. As a result, the optimized focal offsets are a 3.3-fold radius ahead and a 2.0-fold radius behind the trapping laser focus in the forward tracking and the backward tracking, respectively. The experimental result agrees well with a theoretical prediction using the Mie scattering theory.

© 2012 Optical Society of America

OCIS Codes
(040.0040) Detectors : Detectors
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(140.7010) Lasers and laser optics : Laser trapping
(180.3170) Microscopy : Interference microscopy
(290.5850) Scattering : Scattering, particles

ToC Category:
Lasers and Laser Optics

History
Original Manuscript: January 3, 2012
Revised Manuscript: April 25, 2012
Manuscript Accepted: July 9, 2012
Published: August 2, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Ai-Tang Chang, Yi-Ren Chang, Sien Chi, and Long Hsu, "Optimization of probe-laser focal offsets for single-particle tracking," Appl. Opt. 51, 5643-5648 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-23-5643


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