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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 2 — Mar. 4, 2013

Noise reduction in digital lensless holographic microscopy by engineering the light from a light-emitting diode

Jorge Garcia-Sucerquia  »View Author Affiliations


Applied Optics, Vol. 52, Issue 1, pp. A232-A239 (2013)
http://dx.doi.org/10.1364/AO.52.00A232


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Abstract

By engineering the light from a light-emitting diode (LED) the noises present in digital lensless holographic microscopy (DLHM) are reduced. The partially coherent light from an LED is tailored to produce a spherical wavefront with limited coherence time and the spatial coherence needed by DLHM to work. DLHM with this engineered light source is used to image biological samples that cover areas of the order of mm2. The ratio between the diameter of the area that is almost coherently illuminated to the diameter of the illumination area is utilized as parameter to quantify the performance of the DLHM with the engineered LED light source. Experimental results show that while the noises can be reduced effectively the spatial resolution can be kept in the micrometer range.

© 2012 Optical Society of America

OCIS Codes
(030.1670) Coherence and statistical optics : Coherent optical effects
(030.4280) Coherence and statistical optics : Noise in imaging systems
(030.6140) Coherence and statistical optics : Speckle
(110.0180) Imaging systems : Microscopy
(090.1995) Holography : Digital holography

History
Original Manuscript: August 10, 2012
Revised Manuscript: October 6, 2012
Manuscript Accepted: October 15, 2012
Published: November 27, 2012

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Jorge Garcia-Sucerquia, "Noise reduction in digital lensless holographic microscopy by engineering the light from a light-emitting diode," Appl. Opt. 52, A232-A239 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-52-1-A232

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