OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 2 — Mar. 4, 2013

Gradient field microscopy for label-free diagnosis of human biopsies [Invited]

Taewoo Kim, Shamira Sridharan, André Kajdacsy-Balla, Krishnarao Tangella, and Gabriel Popescu  »View Author Affiliations


Applied Optics, Vol. 52, Issue 1, pp. A92-A96 (2013)
http://dx.doi.org/10.1364/AO.52.000A92


View Full Text Article

Enhanced HTML    Acrobat PDF (1161 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We introduce a label-free method for detecting high-grade prostatic intraepithelial neoplasia (HGPIN) in unstained biopsies. We image this condition based on the identification of basal cells in biopsies that otherwise would resemble prostate cancer by unassisted histologic examination. Gradient field microscopy (GFM) is used as a label-free imaging method which increases the contrast of a transparent sample by taking the first-order phase derivative that is very sensitive to rapid refractive index changes. GFM is able to image the basal cell layers in HGPIN biopsies because of their rapid refractive index changes at the periphery of small glandular structures.

© 2012 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.4730) Medical optics and biotechnology : Optical pathology
(170.6935) Medical optics and biotechnology : Tissue characterization

History
Original Manuscript: August 16, 2012
Revised Manuscript: September 26, 2012
Manuscript Accepted: September 30, 2012
Published: October 30, 2012

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Taewoo Kim, Shamira Sridharan, André Kajdacsy-Balla, Krishnarao Tangella, and Gabriel Popescu, "Gradient field microscopy for label-free diagnosis of human biopsies [Invited]," Appl. Opt. 52, A92-A96 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-52-1-A92

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited