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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 2 — Mar. 4, 2013

Fast phase reconstruction in white light diffraction phase microscopy

Hoa V. Pham, Christopher Edwards, Lynford L. Goddard, and Gabriel Popescu  »View Author Affiliations


Applied Optics, Vol. 52, Issue 1, pp. A97-A101 (2013)
http://dx.doi.org/10.1364/AO.52.000A97


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Abstract

In off-axis interferometry, we usually have to deal with the unwrapping process, which is very computationally intensive and prevents us from real time phase reconstruction. The wrapping problem usually occurs when imaging thick objects, which introduce phase shifts of more than 2π radians. However, in off-axis interferometry, the nonzero angle of interference of the two beams creates a ramp in the phase across the image that can produce phase wrapping errors. In this paper, we propose a simple technique that avoids the need for the unwrapping step in reconstructing quantitative phase images in white light diffraction phase microscopy of thin samples. We show that this approach can improve significantly the phase reconstruction speed and allow high impact applications, such as real-time blood testing.

© 2013 Optical Society of America

OCIS Codes
(180.3170) Microscopy : Interference microscopy
(110.5086) Imaging systems : Phase unwrapping
(110.3010) Imaging systems : Image reconstruction techniques

History
Original Manuscript: August 16, 2012
Revised Manuscript: September 13, 2012
Manuscript Accepted: September 26, 2012
Published: October 30, 2012

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Hoa V. Pham, Christopher Edwards, Lynford L. Goddard, and Gabriel Popescu, "Fast phase reconstruction in white light diffraction phase microscopy," Appl. Opt. 52, A97-A101 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-52-1-A97


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References

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