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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 9 — Oct. 2, 2013

Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study

S. Hoda Moosavi, Cristian Gohn-Kreuz, and Alexander Rohrbach  »View Author Affiliations


Applied Optics, Vol. 52, Issue 23, pp. 5835-5842 (2013)
http://dx.doi.org/10.1364/AO.52.005835


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Abstract

Confocal line detection has been shown to improve contrast in light-sheet-based microscopy especially when illuminating the sample by Bessel beams. Besides their self-reconstructing capability, the stability in propagation direction of Bessel beams allows to block the unwanted emission light from the Bessel beam’s ring system. However, due to phase aberrations induced especially at the border of the specimen, Bessel beams may not propagate along lines parallel to the slit detector. Here we present a concept of how to correct the phase of each incident Bessel beam such that the efficiency of confocal line detection is improved by up to 200%–300%. The applicability of the method is verified by the results obtained from numerical simulations based on the beam propagation method.

© 2013 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
(070.3185) Fourier optics and signal processing : Invariant optical fields
(080.5692) Geometric optics : Ray trajectories in inhomogeneous media
(070.7345) Fourier optics and signal processing : Wave propagation
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Microscopy

History
Original Manuscript: June 13, 2013
Revised Manuscript: July 11, 2013
Manuscript Accepted: July 14, 2013
Published: August 9, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Citation
S. Hoda Moosavi, Cristian Gohn-Kreuz, and Alexander Rohrbach, "Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study," Appl. Opt. 52, 5835-5842 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-52-23-5835

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