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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 3 — Apr. 4, 2013

Native fluorescence spectra of human cancerous and normal breast tissues analyzed with non-negative constraint methods

Yang Pu, Wubao Wang, Yuanlong Yang, and Robert R. Alfano  »View Author Affiliations


Applied Optics, Vol. 52, Issue 6, pp. 1293-1301 (2013)
http://dx.doi.org/10.1364/AO.52.001293


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Abstract

The native fluorescence spectra of human cancerous and normal breast tissues were investigated using the selected excitation wavelength of 340 nm to excite key building block molecules, such as reduced nicotinamide adenine dinucleotide (NADH), collagen, and flavin. The measured emission spectra were analyzed using a non-negative constraint method, namely multivariate curve resolution with alternating least-squares (MCR-ALS). The results indicate that the biochemical changes of tissue can be exposed by native fluorescence spectra analysis. The MCR-ALS-extracted components corresponding to the key fluorophores in breast tissue, such as collagen, NADH, and flavin, show differences of relative contents of fluorophores in cancerous and normal breast tissues. This research demonstrates that the native fluorescence spectroscopy measurements are effective for detecting changes of fluorophores composition in tissues due to the development of cancer. Native fluorescence spectroscopy analyzed by MCR-ALS may have the potential to be a new armamentarium.

© 2013 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.0300) Spectroscopy : Spectroscopy
(300.6170) Spectroscopy : Spectra

ToC Category:
Spectroscopy

History
Original Manuscript: October 22, 2012
Revised Manuscript: November 28, 2012
Manuscript Accepted: December 21, 2012
Published: February 18, 2013

Virtual Issues
Vol. 8, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yang Pu, Wubao Wang, Yuanlong Yang, and Robert R. Alfano, "Native fluorescence spectra of human cancerous and normal breast tissues analyzed with non-negative constraint methods," Appl. Opt. 52, 1293-1301 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-52-6-1293

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