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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 3 — Mar. 6, 2014

White light differential interference contrast microscope with a Sagnac interferometer

Sanjib Chatterjee and Y. Pavan Kumar  »View Author Affiliations


Applied Optics, Vol. 53, Issue 2, pp. 296-300 (2014)
http://dx.doi.org/10.1364/AO.53.000296


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Abstract

A new technique for producing a white light differential interference contrast (DIC) image using a lateral shearing, rotation phase shifting Sagnac interferometer (SI) is proposed. The SI, placed in the image space after the tube lens of a microscope system with spatially coherent white light Kohler illumination, splits the image forming beam into coherent components with small lateral shear. Phase shifts, between the interfering components, which can be considered as biased phase difference (BPD), are introduced by applying small angular rotation of the SI in its own plane. This variable BPD between the interfering white light components produces a uniform intensity colored background. The object related phase shift, due to the height difference between two close points on the object surface with separation on the order of least resolvable separation of the microscope objective, in addition to the BPD would produce a change in intensity/hue/color against a uniform background due to the BPD. Thus a DIC image is formed and the variable BPD provides an excellent means of improving the contrast of the image.

© 2014 Optical Society of America

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(120.6650) Instrumentation, measurement, and metrology : Surface measurements, figure

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: September 4, 2013
Revised Manuscript: December 11, 2013
Manuscript Accepted: December 11, 2013
Published: January 10, 2014

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Sanjib Chatterjee and Y. Pavan Kumar, "White light differential interference contrast microscope with a Sagnac interferometer," Appl. Opt. 53, 296-300 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-53-2-296

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