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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Phase-stepping technique for highly sensitive microscopic surface plasmon resonance biosensor

Chonglei Zhang, Rong Wang, Yijia Wang, Siwei Zhu, Changjun Min, and X.-C. Yuan  »View Author Affiliations

Applied Optics, Vol. 53, Issue 5, pp. 836-840 (2014)

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In this paper, the phase-stepping technique is applied to improve a phase-sensitive surface plasmon resonance biosensor based on differential interferometry between focused radially polarized and azimuthally polarized cylindrical vector beams. Detailed analysis is presented for the phase-stepping method, and the least squares unwrapping algorithm is employed to detect the phase distribution in correspondence to the refractive index of sample. Benefiting from the phase-stepping technique, both the measurement speed and sensitivity are improved significantly. The proposed sensor maintains high sensitivity of 9.4×107RIU/1° and a wide dynamic range of 0.35 RIU simultaneously. Furthermore, the real-time binding reaction process of bovine serum albumin with antibody is monitored to verify the system for potential biological applications.

© 2014 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(240.6680) Optics at surfaces : Surface plasmons
(280.4788) Remote sensing and sensors : Optical sensing and sensors
(100.5088) Image processing : Phase unwrapping

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: October 7, 2013
Revised Manuscript: December 26, 2013
Manuscript Accepted: December 28, 2013
Published: February 5, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Chonglei Zhang, Rong Wang, Yijia Wang, Siwei Zhu, Changjun Min, and X.-C. Yuan, "Phase-stepping technique for highly sensitive microscopic surface plasmon resonance biosensor," Appl. Opt. 53, 836-840 (2014)

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