This paper evaluates two multivariate strategies for classifying near-infrared (NIR) spectroscopic data for the detection of animal by-product meals (henceforth generically termed AbP) as an ingredient in compound feedingstuffs. Classification models were developed to discriminate between the presence and absence of animal-origin meals in compound feeds using two forms of discriminant partial least squares (PLS) regression: the algorithms PLS1 and PLS2. The training set comprised 433 commercial feeds, of which 148 contained AbP and the other 285 were stated to be AbP-free. Since the initial set contained unequal numbers of each class, the effect of this imbalance was analyzed by applying the same algorithms to a training set containing equal numbers of AbP-free and AbP-containing samples. The best classification model (97.42% of samples correctly classified), obtained with PLS2, that showed less sensitivity to the use of class-unbalanced sets, was externally validated using a set of 18 samples (10 AbP-containing and 8 AbP-free); all samples were correctly classified, except for one AbP-free sample that was classified as containing AbP (false positive). The results suggest that the application of PLS discriminant analysis to NIR spectroscopic data enables detection of AbP, a feed ingredient banned since the bovine spongiform encephalopathy (BSE) crisis; this confirms the value of NIRS qualitative analysis for product authentication purposes.
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics
D. C. Pérez-Marín, A. Garrido-Varo, and J. E. Guerrero, "Optimization of Discriminant Partial Least Squares Regression Models for the Detection of Animal By-Product Meals in Compound Feedingstuffs by Near-Infrared Spectroscopy," Appl. Spectrosc. 60, 1432-1437 (2006)
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