OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

A Chromatographic Approach for Fluorescence Reduction in Liquid Raman Analysis

Nils Kristian Afseth, Vegard Herman Segtnan, Jens Petter Wold, and Brian J. Marquardt

Applied Spectroscopy, Vol. 61, Issue 12, pp. 1283-1289 (2007)


View Full Text Article

Acrobat PDF (667 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

In this study a chromatographic approach for fluorescence reduction in liquid Raman analysis has been evaluated. The idea behind the approach is to apply a chromatographic separation step prior to Raman analysis in order to separate fluorescing compounds from other components of interest, thus facilitating better quantitative and qualitative analysis of the latter components. A real-time liquid-core Raman waveguide detector designed for chromatographic applications was used in the study, thus providing real-time chemical pretreatment of liquid samples for Raman analysis. Twenty aqueous mixtures of additives frequently found in beverages were analyzed, and for comparative purposes the mixtures were also analyzed in the Raman waveguide detector without chromatographic separation and with a conventional immersion probe. Both qualitatively and quantitatively satisfying results were obtained using the chromatographic Raman approach, and the technique provided possibilities for quantitative and qualitative assessments superior to the two other instrumental setups. The technique may provide additional benefits through sensitivity enhancements, and the approach is simple, inexpensive, and easy to implement in the average applied Raman laboratory. The analysis of various chemical systems and factors such as system stability over time need further evaluation in order to confirm the general applicability of the approach.

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Nils Kristian Afseth, Vegard Herman Segtnan, Jens Petter Wold, and Brian J. Marquardt, "A Chromatographic Approach for Fluorescence Reduction in Liquid Raman Analysis," Appl. Spectrosc. 61, 1283-1289 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-61-12-1283

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited