Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 61,
  • Issue 5,
  • pp. 465-469
  • (2007)

Temperature-Controlled Confocal Raman Microscopy to Detect Phase Transitions in Phospholipid Vesicles

Not Accessible

Your library or personal account may give you access

Abstract

Optical-trapping confocal Raman microscopy enhances the capabilities of traditional Raman spectroscopy for the analysis of small particles by significantly reducing the sampling volume and minimizing background signal from the particle surroundings. Chemical composition and structural information can be obtained from optically trapped particles in aqueous solution without the need for labeling or extensive sample preparation. In this work, the challenges of measuring temperature dependent changes in suspended particles are addressed with the development of a small-volume, thermally conductive sample cell attached to a temperature-controlled microscope stage. To demonstrate its function, the gel to liquid-crystalline phase transitions of optically trapped lipid vesicles, composed of pure 1,2-ditridecanoyl-<i>sn</i>-glycero-3-phosphocholine (DTPC), 1,2-dimyristoyl-<i>sn</i>-glycero-3-phosphocholine (DMPC), and 1,2-dipalmitoyl-<i>sn</i>-glycero-3-phosphocholine (DPPC), were detected by changes in Raman spectra of the lipid bilayer. The Raman scattering data were found to correlate well with differential scanning calorimetry (DSC) results.

PDF Article
More Like This
Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy

Tze-Hsuan Liu, Jian-Long Xiao, Chau-Hwang Lee, and Jiunn-Yuan Lin
Appl. Opt. 50(19) 3311-3315 (2011)

Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures

Mateo R. Hernandez, Elyse N. Towns, Terry C. Ng, Brian C. Walsh, Richard Osibanjo, Atul N. Parikh, and Donald P. Land
Appl. Opt. 51(15) 2842-2846 (2012)

Visualization and measurement of the local absorption coefficients of single bilayer phospholipid membranes using scanning near-field optical microscopy

Arif M. Siddiquee, Imad Younus Hasan, Shibiao Wei, Daniel Langley, Eugeniu Balaur, Chen Liu, Jiao Lin, Brian Abbey, Adam Mechler, and Shanshan Kou
Biomed. Opt. Express 10(12) 6569-6579 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved