A small endoscope system equipped with a micro Raman probe is developed for <i>in vivo</i> Raman measurements in living rats. The measurements are done under anesthesia and artificial respiration to minimize the impact on the rats. Raman spectra of living rat esophagus and stomach are successfully measured. Our results suggest that the Raman spectra reflect subsurface tissue structure that cannot be distinguished in the endoscope image. After the experiments, rats recover without any aftereffects. It is verified that the Raman measurement using the present system is safe and noninvasive for rats.
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics
Yusuke Hattori, Yuichi Komachi, Toru Asakura, Toru Shimosegawa, Gen-Ichi Kanai, Hideo Tashiro, and Hidetoshi Sato, "In Vivo Raman Study of the Living Rat Esophagus and Stomach Using a Micro-Raman Probe Under an Endoscope," Appl. Spectrosc. 61, 579-584 (2007)