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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

A Sensitive Chemiluminescence Procedure for the Determination of Carbon Monoxide with Myoglobin–Luminol Chemiluminescence System

Xiaofeng Xie, Xili He, and Zhenghua Song

Applied Spectroscopy, Vol. 61, Issue 7, pp. 706-710 (2007)


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Abstract

A novel chemiluminescence method combined with the flow injection technique for the determination of carbon monoxide is presented in this paper. The chemiluminescence signal based on the reaction between myoglobin and luminol in an alkaline medium was remarkably enhanced by carbon monoxide. The enhanced chemiluminescence intensity was linear with carbon monoxide concentration in the range from 0.01 to 10.0 pmol·L−1, and the detection limit was 3 × 10−3 pmol·L−1 (3σ). The whole process, including sampling and washing, could be completed in 0.5 min with a relative standard deviation of less than 4.0%. The proposed method was applied successfully in the assay of carbon monoxide in human serum and artificial water samples without any pretreatment procedure.

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Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Xiaofeng Xie, Xili He, and Zhenghua Song, "A Sensitive Chemiluminescence Procedure for the Determination of Carbon Monoxide with Myoglobin–Luminol Chemiluminescence System," Appl. Spectrosc. 61, 706-710 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-61-7-706


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