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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Sheath-Flow Cuvette for High-Sensitivity Laser-Induced Fluorescence Detection in Capillary Electrophoresis

Kimia Sobhani, David A. Michels, and Norman J. Dovichi

Applied Spectroscopy, Vol. 61, Issue 7, pp. 777-779 (2007)


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Abstract

The sheath-flow cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3σ) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.

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Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Kimia Sobhani, David A. Michels, and Norman J. Dovichi, "Sheath-Flow Cuvette for High-Sensitivity Laser-Induced Fluorescence Detection in Capillary Electrophoresis," Appl. Spectrosc. 61, 777-779 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-61-7-777


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