OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 12 — Dec. 1, 2008

Attenuated Total Reflection–Fourier Transform Infrared Imaging of Large Areas Using Inverted Prism Crystals and Combining Imaging and Mapping

K. L. Andrew Chan and Sergei G. Kazarian

Applied Spectroscopy, Vol. 62, Issue 10, pp. 1095-1101 (2008)


View Full Text Article

Acrobat PDF (2284 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Attenuated total reflection–Fourier transform infrared (ATR-FT-IR) imaging is a very useful tool for capturing chemical images of various materials due to the simple sample preparation and the ability to measure wet samples or samples in an aqueous environment. However, the size of the array detector used for image acquisition is often limited and there is usually a trade off between spatial resolution and the field of view (FOV). The combination of mapping and imaging can be used to acquire images with a larger FOV without sacrificing spatial resolution. Previous attempts have demonstrated this using an infrared microscope and a Germanium hemispherical ATR crystal to achieve images of up to 2.5 mm × 2.5 mm but with varying spatial resolution and depth of penetration across the imaged area. In this paper, we demonstrate a combination of mapping and imaging with a different approach using an external optics housing for large ATR accessories and inverted ATR prisms to achieve ATR-FT-IR images with a large FOV and reasonable spatial resolution. The results have shown that a FOV of 10 mm × 14 mm can be obtained with a spatial resolution of approximately 40–60 μm when using an accessory that gives no magnification. A FOV of 1.3 mm × 1.3 mm can be obtained with spatial resolution of approximately 15–20 μm when using a diamond ATR imaging accessory with 4× magnification. No significant change in image quality such as spatial resolution or depth of penetration has been observed across the whole FOV with this method and the measurement time was approximately 15 minutes for an image consisting of 16 image tiles.

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
K. L. Andrew Chan and Sergei G. Kazarian, "Attenuated Total Reflection–Fourier Transform Infrared Imaging of Large Areas Using Inverted Prism Crystals and Combining Imaging and Mapping," Appl. Spectrosc. 62, 1095-1101 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-62-10-1095

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited