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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 12 — Dec. 1, 2008

Evaluation of Femtosecond Laser-Induced Breakdown Spectroscopy for Analysis of Animal Tissues

Dário Santos, Ricardo Elgul Samad, Lílian Cristina Trevizan, Anderson Zanardi de Freitas, Nilson Dias Vieira, and Francisco José Krug

Applied Spectroscopy, Vol. 62, Issue 10, pp. 1137-1143 (2008)


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Abstract

The aim of this work was to evaluate the performance of femtosecond laser-induced breakdown spectroscopy (fs-LIBS) for the determination of elements in animal tissues. Sample pellets were prepared from certified reference materials, such as liver, kidney, muscle, hepatopancreas, and oyster, after cryogenic grinding assisted homogenization. Individual samples were placed in a two-axis computer-controlled translation stage that moved in the plane orthogonal to a beam originating from a Ti:Sapphire chirped-pulse amplification (CPA) laser system operating at 800 nm and producing a train of 840 μJ and 40 fs pulses at 90 Hz. The plasma emission was coupled into the optical fiber of a high-resolution intensified charge-coupled device (ICCD)–echelle spectrometer. Time-resolved characteristics of the laser-produced plasmas showed that the best results were obtained with delay times between 80 and 120 ns. Data obtained indicate both that it is a matrix-independent sampling process and that fs-LIBS can be used for the determination of Ca, Cu, Fe, K, Mg, Na, and P, but efforts must be made to obtain more appropriate detection limits for Al, Sr, and Zn.

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Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Dário Santos, Ricardo Elgul Samad, Lílian Cristina Trevizan, Anderson Zanardi de Freitas, Nilson Dias Vieira, and Francisco José Krug, "Evaluation of Femtosecond Laser-Induced Breakdown Spectroscopy for Analysis of Animal Tissues," Appl. Spectrosc. 62, 1137-1143 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-62-10-1137


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