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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 4 — Apr. 23, 2008

A Multi-Source Portable Light Emitting Diode Spectrofluorometer

Safwan Obeidat, Baolong Bai, Gary D. Rayson, Dean M. Anderson, Adam D. Puscheck, Serge Y. Landau, and Tzach Glasser

Applied Spectroscopy, Vol. 62, Issue 3, pp. 327-332 (2008)


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Abstract

A portable luminescence spectrofluorometer weighing only 1.5 kg that uses multiple light emitting diodes (LEDs) as excitation sources was developed and evaluated. Excitation using a sequence of seven individual broad-band LED emission sources enabled the generation of excitation-emission spectra using a light weight (<1.5 kg) spectrometer. Limits of detection for rhodamine 6G, rhodamine B, and fluorescein were 2.9, 3.2, and 11.0 nM, respectively. Generation of excitation-emission matrices (EEMs) enabled the analysis of samples containing mixtures of rhodamine B and fluorescein. Buffered saline plant and animal feed extracts were also analyzed using this instrument. These samples included the woody plants <i>Pistacia lentiscus</i> (Evergreen pistache or Mastic) and <i>Philyria latifolia</i>, and the herbaceous species <i>Medicago sativa</i> (alfalfa), <i>Trifolium spp</i>. (clover), and a feed concentrate. Application of multi-way principal component analysis (MPCA) to the resulting three-dimensional data sets enabled discernment among these various diet constituents.

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Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Safwan Obeidat, Baolong Bai, Gary D. Rayson, Dean M. Anderson, Adam D. Puscheck, Serge Y. Landau, and Tzach Glasser, "A Multi-Source Portable Light Emitting Diode Spectrofluorometer," Appl. Spectrosc. 62, 327-332 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-62-3-327


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