We studied the collisional quenching of the erythrosine B fluorophore by potassium iodide. The quenching follows a Stern–Volmer dependence up to the highest quencher concentration. The lifetime of erythrosine B decreases to 24 ps in 5.02 M of potassium iodide. The quantum yield of erythrosine B in the presence of 5.02 M KI is 0.0035. The relatively high brightness makes this compound attractive as an ultrashort reference in time-resolved measurements. In both frequency- and time-domain fluorescence techniques, there is a need for lifetime standards with extremely short decay times. Mimicking the instantaneous scattering at longer wavelengths allows color-effect-free measurements in the emission region. Another motivation is the problem of obtaining the impulse response function in the case of two-photon excitation. Time-resolved microscopy also benefits from fast-decaying dyes because the impulse response function can be evaluated at the emission wavelength of the investigated specimen without changing filters. We demonstrated that impulse response functions for commonly used detectors are practically the same for scattering as for quenched erythrosine B emission. We also analyzed a complex fluorescence decay using both elastic scattering and quenched erythrosine B emission as a response function.
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics
Mariusz Szabelski, Douglas Ilijev, Pabak Sarkar, Rafal Luchowski, Zygmunt Gryczynski, Peter Kapusta, Rainer Erdmann, and Ignacy Gryczynski, "Collisional Quenching of Erythrosine B as a Potential Reference Dye for Impulse Response Function Evaluation," Appl. Spectrosc. 63, 363-368 (2009)
References are not available for this paper.
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.