The use of trichloroacetyl isocyanate (TAI) to mark both hydroxyl and carboxyl groups borne by the hydrolysis or methanolysis of suberin fragments (a complex mixture of hydroxy fatty acids), allowed the quantitative assessment of the ratio between carboxyl and hydroxy groups, as well as the ratio between primary and secondary hydroxy groups, to be carried out reliably by <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy. All the samples thus analyzed displayed an excess of CO<sub>2</sub>H (or CO<sub>2</sub>CH<sub>3</sub>) functions with respect to the OH counterparts, albeit to a variable extent, depending on the procedure adopted to isolate the suberin fragments. The precise knowledge of the molar ratio of these two reactive moieties is fundamental for the correct utilization of suberin monomers in polymerization reactions leading to aliphatic polyesters.
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics
Andreia F. Sousa, Alessandro Gandini, Armando J. D. Silvestre, and Carlos Pascoal Neto, "Determination of the Hydroxy and Carboxylic Acid Groups in Natural Complex Mixtures of Hydroxy Fatty Acids by 1H Nuclear Magnetic Resonance Spectroscopy," Appl. Spectrosc. 63, 873-878 (2009)
References are not available for this paper.
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.