OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 11 — Oct. 21, 2009

A Comparison of Pseudomonas aeruginosa Biofilm Development on ZnSe and TiO2 Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy

Jonathan W. D. Comeau, Judith Pink, Evan Bezanson, Colin D. Douglas, David Pink, and Truis Smith-Palmer

Applied Spectroscopy, Vol. 63, Issue 9, pp. 1000-1007 (2009)


View Full Text Article

Acrobat PDF (790 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

The growth of Pseudomonas aeruginosa PAO1 biofilms on ZnSe internal reflection elements (IREs) was compared with their growth on TiO2-coated ZnSe over several days using attenuated total reflection Fourier transform infrared (ATR-FT-IR) spectroscopy. The effect of the TiO2 coating on the IR spectra of reference compounds and cell suspensions was determined to aid in the interpretation of the data. The presence of TiO2 on the surface of a ZnSe IRE tripled the size of the amide II peak and facilitated the detection of pyoverdin production due to its increased adsorption on the coated surface. A 50% increase in the length of the lag phase was observed for PAO1 growth on TiO2-coated surfaces as compared to growth on ZnSe. Biofilms on both surfaces exhibited a growth maximum for all components, followed by restructuring at the surface characterized by a decrease in the signal. The composition of biofilms grown on TiO2 was relatively constant after the restructuring phase, while the extracellular polymeric substance (EPS) component of the biofilms grown on ZnSe gradually increased. The peak due to the carbohydrate component of EPS was much larger in the spectra of biofilms than in those of planktonic cells. The increase of the pyoverdin signal over time in the spectra of the biofilms on TiO2 closely followed the overall increase in biomass. However, no signal from pyoverdin was detected in the presence of ferric ions.

Virtual Issues
Vol. 4, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Jonathan W. D. Comeau, Judith Pink, Evan Bezanson, Colin D. Douglas, David Pink, and Truis Smith-Palmer, "A Comparison of Pseudomonas aeruginosa Biofilm Development on ZnSe and TiO2 Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy," Appl. Spectrosc. 63, 1000-1007 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-63-9-1000

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited