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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 11 — Oct. 21, 2009

A Two-Layer Gold Surface with Improved Surface Enhancement for Spectro-Electrochemistry Using Surface-Enhanced Infrared Absorption Spectroscopy

C. Nowak, C. Luening, W. Knoll, and R. L. C. Naumann

Applied Spectroscopy, Vol. 63, Issue 9, pp. 1068-1074 (2009)


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Abstract

A two-layer gold surface is developed for use with surface-enhanced infrared absorption spectroscopy (SEIRAS) consisting of a conducting underlayer onto which Au nanoparticles (AuNPs) are grown by self-catalyzed electroless deposition. AuNPs are grown on protruding substructures of the 25 nm thin underlayer. The enhancement factor of the two-layer gold surface is controlled by the growth conditions. Cytochrome c adsorbed to a self-assembled monolayer of mercaptoethanol is used as a reference system. Under optimum conditions the absorbance of the amide I band is increased by a factor of 5 versus the classical SEIRAS surface. Reversible reduction/oxidation of cytochrome c on the two-layer gold surface is shown to take place by cyclic voltammetry.

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Vol. 4, Iss. 11 Virtual Journal for Biomedical Optics

Citation
C. Nowak, C. Luening, W. Knoll, and R. L. C. Naumann, "A Two-Layer Gold Surface with Improved Surface Enhancement for Spectro-Electrochemistry Using Surface-Enhanced Infrared Absorption Spectroscopy," Appl. Spectrosc. 63, 1068-1074 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-63-9-1068

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