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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 1 — Jan. 3, 2011

Far-Infrared Spectroscopy of Protein Higher-Order Structures

Robert J. Falconer, Hidayatul A. Zakaria, Yuan Y. Fan, Andrew P. Bradley, and Anton P.J. Middelberg

Applied Spectroscopy, Vol. 64, Issue 11, pp. 1259-1264 (2010)


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Abstract

Far-infrared (FIR) spectroscopy in the spectral region of 50–450 cm<sup>–1</sup> was used to study a series of protein higher-order structures constructed using β-lactoglobulin and polyomavirus capsid protein VP1. There were marked differences in the spectra for β-lactoglobulin monomer and dimer and between untreated β-lactoglobulin and heat-induced gels formed at neutral pH. Untreated β-lactoglobulin and heat-induced gels formed at acidic pH exhibited little difference in their spectra. Assembly of the quaternary structure of polyomavirus virus-like particles also caused large changes in the FIR spectra. These findings suggest that FIR spectroscopy may prove useful in studying some protein quaternary and higher-order structures. There was evidence of detection of β-lactoglobulin dimerization, intermolecular disulfide bonding in heat-induced neutral gels, and polyomavirus virus-like particle assembly but no evidence that FIR could detect β-lactoglobulin fibrils with their polymeric structure and hydrogen-bonded intermolecular β-pleated sheeting. Index Headings: Terahertz; Beta-lactoglobulin; β-lactoglobulin; Amyloid; Fibril; Polyomavirus; Virus-like particle; Quaternary structure; Vibrational spectrometry.

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Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Robert J. Falconer, Hidayatul A. Zakaria, Yuan Y. Fan, Andrew P. Bradley, and Anton P.J. Middelberg, "Far-Infrared Spectroscopy of Protein Higher-Order Structures," Appl. Spectrosc. 64, 1259-1264 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-64-11-1259


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