We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying single-cell dynamics of cell populations and for the development of high-throughput Raman based cytometers.
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics
Rui Liu, Douglas S. Taylor, Dennis L. Matthews, and James W. Chan, "Parallel Analysis of Individual Biological Cells Using Multifocal Laser Tweezers Raman Spectroscopy," Appl. Spectrosc. 64, 1308-1310 (2010)