OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Molecular Fluorescence Enhancement on Fractal-Like Structures

Rafal Luchowski, Tanya Shtoyko, Evgenia Matveeva, Pabak Sarkar, Julian Borejdo, Zygmunt Gryczynski, and Ignacy Gryczynski

Applied Spectroscopy, Vol. 64, Issue 6, pp. 578-583 (2010)


View Full Text Article

Acrobat PDF (641 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

In this report we discuss strong fluorescence enhancements on electrochemically grown silver nanostructures examined through fluorescence lifetime imaging microscopy (FLIM). Silver fractal-like nanostructures were deposited on three different substrates: glass, plastic, and silicon. For all of the surfaces the same dye was tested, DyLight 649, deposited in the form of a model immunoassay through excitation from a 635 nm pulsed solid-state laser. The brightness improvement in hot spots exceeded 300 fold, which is about two times higher than was observed previously on similar surfaces. The strongest enhancements correspond to the shortest lifetimes, indicating a strong interaction between excited molecules and silver nanostructures. Additionally, the photostability of the fluorescence dye was dramatically increased in the presence of electrochemically deposited silver nanostructures. The production of silver fractals is easy, very controllable, and can be applied to any surface. We therefore believe that silver fractal-like nanostructures can be used successfully in ultrasensitive assays and fluorophore trace detection.

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Rafal Luchowski, Tanya Shtoyko, Evgenia Matveeva, Pabak Sarkar, Julian Borejdo, Zygmunt Gryczynski, and Ignacy Gryczynski, "Molecular Fluorescence Enhancement on Fractal-Like Structures," Appl. Spectrosc. 64, 578-583 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-64-6-578

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited