OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

Monte Carlo Simulation of Fluorescence Spectra of Normal and Dysplastic Cervical Tissues for Optimizing Excitation/Receiving Arrangements

Shou Chia Chu and Huihua Kenny Chiang

Applied Spectroscopy, Vol. 64, Issue 7, pp. 708-713 (2010)

View Full Text Article

Acrobat PDF (18672 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

  • Export Citation/Save Click for help


Recent studies have demonstrated that the autofluorescence technique is a very promising tool for early-stage cancer diagnosis in various tissues. Many researchers have applied the autofluorescence technique through fiber-optic cables to excite tissues and collect the fluorescent emission signal from the tissues for discrimination analysis. In this study, we developed a Monte Carlo–light-induced autofluorescence (MCS LIAF) simulation model to optimize the oblique angle in the excitation optical fiber and the spatial arrangements in the receiving optical fiber. Our aim was to discriminate cervical tissues at different dysplastic stages. The model combined the structure of multi-layered tissues, tissue optical scattering and absorption parameters, tissue fluorophore concentration, the characteristic fluorescent spectrum of fluorophores, and the excitation and receiving arrangement of the optical fibers. The results show that the optimal oblique angle of the excitation optical fiber is between 0° and 45° and that the optimal receiving optical fiber is positioned 200 μm away from the origin. We also observed that changing the excitation angle is very useful in differentiating normal from cervical intraepithelial neoplasia (CIN) I or CIN II tissues. Also, using the fluorescence peak ratio of NADH/collagen can help discriminate CIN III from normal tissues and CIN I/II tissues.

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Shou Chia Chu and Huihua Kenny Chiang, "Monte Carlo Simulation of Fluorescence Spectra of Normal and Dysplastic Cervical Tissues for Optimizing Excitation/Receiving Arrangements," Appl. Spectrosc. 64, 708-713 (2010)

Sort:  Journal  |  Reset


References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited