OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

Determination of Temporal and Spatial Concentration Gradients in Hydrogel Beads Using Multiphoton Microscopy Techniques

Tilman Schwendt, Claas Michalik, Michael Zavrel, Alexander Dennig, Antje C. Spiess, Reinhart Poprawe, and Christoph Janzen

Applied Spectroscopy, Vol. 64, Issue 7, pp. 720-726 (2010)


View Full Text Article

Acrobat PDF (2887 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Multiphoton microscopy is a promising technique to detect spatially and temporally resolved concentration gradients of chemical compounds, e.g., reactants in hydrogel-encapsulated biocatalysts. In contrast to current techniques, the improved spatial and temporal resolution of this method in data acquisition and its ability to measure hydrogel beads facilitates the identification of various kinetic phenomena. To our knowledge, multiphoton microscopy is used here for the first time to examine diffusion, mass transfer, and reaction in immobilized hydrogel systems. In a first step, the phenomena of diffusion and diffusion-coupled mass transfer through the phase interface are investigated in the bead center. Finally, the complete system—consisting of diffusion, mass transfer, and enzymatic reaction—is observed by measuring concentration gradients along the bead radius with temporal and spatial resolution. This metrology enables a subsequent mechanistic model identification, which in turn leads to an enhanced knowledge of reaction kinetics and supports the design of biotechnological processes. This task was only possible due to excellent spatial (25 μm) and temporal (5 s) resolution and the accuracy (±1%) achieved by using a multiphoton microscopy setup.

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Tilman Schwendt, Claas Michalik, Michael Zavrel, Alexander Dennig, Antje C. Spiess, Reinhart Poprawe, and Christoph Janzen, "Determination of Temporal and Spatial Concentration Gradients in Hydrogel Beads Using Multiphoton Microscopy Techniques," Appl. Spectrosc. 64, 720-726 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-64-7-720

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited