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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Low-Frequency Spectroscopic Analysis of Monomeric and Fibrillar Lysozyme

Hidayatul A. Zakaria, Bernd M. Fischer, Andrew P. Bradley, Inke Jones, Derek Abbott, Anton P. J. Middelberg, and Robert J. Falconer

Applied Spectroscopy, Vol. 65, Issue 3, pp. 260-264 (2011)


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Abstract

Terahertz time-domain spectroscopy (THz-TDS) and Fourier transform infrared (FT-IR) spectroscopy were used to generate far-infrared and low-frequency spectral measurements of monomeric lysozyme and lysozyme fibrils. The formation of lysozyme fibrils was verified by the Thioflavin T assay and transmission electron microscopy (TEM). It was evident in the FT-IR spectra that between 150 and 350 cm−1 the two spectra diverge, with the lysozyme fibrils showing higher absorbance intensity than the monomeric form. The broad absorption phenomenon is likely due to light scattered from the fibrillar architecture of lysozyme fibrils as supported by simulation of Rayleigh light scattering. The lack of discrete phonon-like peaks suggest that far-infrared spectroscopy cannot detect vibrational modes between the highly ordered hydrogen-bonded beta-pleated sheets of the lysozyme subunit.

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Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Hidayatul A. Zakaria, Bernd M. Fischer, Andrew P. Bradley, Inke Jones, Derek Abbott, Anton P. J. Middelberg, and Robert J. Falconer, "Low-Frequency Spectroscopic Analysis of Monomeric and Fibrillar Lysozyme," Appl. Spectrosc. 65, 260-264 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-65-3-260

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