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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 12 — Dec. 19, 2012

Deep-Ultraviolet (UV) Resonance Raman Spectroscopy as a Tool for Quality Control of Formulated Therapeutic Proteins

Sergey Arzhantsev, Vincent Vilker, and John Kauffman

Applied Spectroscopy, Vol. 66, Issue 11, pp. 1262-1268 (2012)


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Abstract

A deep-ultraviolet (UV) Raman spectrometer with excitation source tunable from 193 to 210 nm has been built and characterized. The dispersion of the spectrometer over the entire range was measured and described theoretically. The relative sensitivity of the spectrometer was estimated using the integrated intensity ratio of two Raman bands of cyclohexane. Resonance Raman spectra of three formulated insulin products were measured and compared. A band-targeted entropy minimization algorithm was applied to the collected spectra for mixture analysis of insulin products. We conclude that it is feasible to develop robust qualitative methods for quality control of protein-based formulated drug using DUVRR spectroscopy.

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Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Sergey Arzhantsev, Vincent Vilker, and John Kauffman, "Deep-Ultraviolet (UV) Resonance Raman Spectroscopy as a Tool for Quality Control of Formulated Therapeutic Proteins," Appl. Spectrosc. 66, 1262-1268 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-66-11-1262

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