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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis

Roger W. Jones, Tonu Reinot, Ursula K. Frei, Yichia Tseng, Thomas Lübberstedt, and John F. McClelland

Applied Spectroscopy, Vol. 66, Issue 4, pp. 447-450 (2012)


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Abstract

Samples of haploid and hybrid seed from three different maize donor genotypes after maternal haploid induction were used to test the capability of automated near-infrared transmission spectroscopy to individually differentiate haploid from hybrid seeds. Using a two-step chemometric analysis in which the seeds were first classified according to genotype and then the haploid or hybrid status was determined proved to be the most successful approach. This approach allowed 11 of 13 haploid and 25 of 25 hybrid kernels to be correctly identified from a mixture that included seeds of all the genotypes.

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Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Roger W. Jones, Tonu Reinot, Ursula K. Frei, Yichia Tseng, Thomas Lübberstedt, and John F. McClelland, "Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis," Appl. Spectrosc. 66, 447-450 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-66-4-447

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