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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 6 — Jun. 27, 2013

Spectral Study and Protein Labeling of Inclusion Complex Between Dye and Calixarene Sulfonate

Xuening Fei, Yong Zhang, Sen Zhu, Lijuan Liu, and Lu Yu

Applied Spectroscopy, Vol. 67, Issue 5, pp. 520-525 (2013)


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Abstract

The host-guest inclusion complex of calix[6]arene sulfonate (SCA6) with thiazole orange (TO) formed in aqueous solution was studied. Absorption and fluorescence techniques were used for the analysis of this inclusion complex. The addition of calixarene sulfonate leads to a decrease in both absorption and fluorescence intensity of the dye, indicating that the inclusion complex was formed. Simultaneously, the inclusion phenomenon of another cyanine dye, Cy3, with calixarene sulfonate was investigated. The stability constant of the two complexes was determined, and the results were compared. The water solubility of TO dye was increased in the presence of calixarene sulfonate, and further protein labeling experiments suggested that this TO-SCA6 complex can act as a fluorescent probe for labeling of biomolecules.

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Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Xuening Fei, Yong Zhang, Sen Zhu, Lijuan Liu, and Lu Yu, "Spectral Study and Protein Labeling of Inclusion Complex Between Dye and Calixarene Sulfonate," Appl. Spectrosc. 67, 520-525 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-67-5-520

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