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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 7 — Aug. 1, 2013

Taxonomic Classification of Phytoplankton with Multivariate Optical Computing, Part I: Design and Theoretical Performance of Multivariate Optical Elements

Joseph A. Swanstrom, Laura S. Bruckman, Megan R. Pearl, Michael N. Simcock, Kathleen A. Donaldson, Tammi L. Richardson, Timothy J. Shaw, and Michael L. Myrick

Applied Spectroscopy, Vol. 67, Issue 6, pp. 620-629 (2013)


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Abstract

Phytoplankton are single-celled, photosynthetic algae and cyanobacteria found in all aquatic environments. Differential pigmentation between phytoplankton taxa allows use of fluorescence excitation spectroscopy for discrimination and classification. For this work, we applied multivariate optical computing (MOC) to emulate linear discriminant vectors of phytoplankton fluorescence excitation spectra by using a simple filter-fluorometer arrangement. We grew nutrient-replete cultures of three differently pigmented species: the coccolithophore Emiliania huxleyi, the diatom Thalassiosira pseudonana, and the cyanobacterium Synechococcus sp. Linear discriminant analysis (LDA) was used to determine a suitable set of linear discriminant functions for classification of these species over an optimal wavelength range. Multivariate optical elements (MOEs) were then designed to predict the linear discriminant scores for the same calibration spectra. The theoretical performance specifications of these MOEs are described.

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Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Joseph A. Swanstrom, Laura S. Bruckman, Megan R. Pearl, Michael N. Simcock, Kathleen A. Donaldson, Tammi L. Richardson, Timothy J. Shaw, and Michael L. Myrick, "Taxonomic Classification of Phytoplankton with Multivariate Optical Computing, Part I: Design and Theoretical Performance of Multivariate Optical Elements," Appl. Spectrosc. 67, 620-629 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-67-6-620


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