OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 7 — Aug. 1, 2013

Taxonomic Classification of Phytoplankton with Multivariate Optical Computing, Part III: Demonstration

Megan R. Pearl, Joseph A. Swanstrom, Laura S. Bruckman, Tammi L. Richardson, Timothy J. Shaw, Heidi M. Sosik, and Michael L. Myrick

Applied Spectroscopy, Vol. 67, Issue 6, pp. 640-647 (2013)


View Full Text Article

Acrobat PDF (543 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

We describe the automatic analysis of fluorescence tracks of phytoplankton recorded with a fluorescence imaging photometer. The optical components and construction of the photometer were described in Part I and Part II of this series in this issue. An algorithm first isolates tracks corresponding to a single phytoplankter transit in the nominal focal plane of a flow cell. Then, the fluorescence streaks in the track that correspond to individual optical elements on the filter wheel are identified. The fluorescence intensity of each streak is integrated and used to calculate ratios. This approach was tested using 853 fluorescence measurements of the coccolithophore Emiliania huxleyi and the diatom Thalassiosira pseudonana. Average intensity ratios for the two classes closely follow those predicted in Part I of this series, with a distribution of ratios in each class that is consistent with the signal-to-noise ratio calculations in Part II for single cells. No overlap of the two class ratios was observed, yielding perfect classification.

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Megan R. Pearl, Joseph A. Swanstrom, Laura S. Bruckman, Tammi L. Richardson, Timothy J. Shaw, Heidi M. Sosik, and Michael L. Myrick, "Taxonomic Classification of Phytoplankton with Multivariate Optical Computing, Part III: Demonstration," Appl. Spectrosc. 67, 640-647 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-67-6-640


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited