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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

Flow-Injection Post-Chemiluminescence Method for the Determination of Palmatine

Suqin Han, Bei Wei, and Fang Hao

Applied Spectroscopy, Vol. 67, Issue 7, pp. 747-751 (2013)

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A post-chemiluminescence (PCL) phenomenon was observed when palmatine was injected in a mixture of N-bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF) after the chemiluminescence (CL) reaction of NBS-alkaline DCF had finished. Based on the PCL reaction, a rapid and sensitive method for the determination of palmatine was established. Under optimum conditions, the CL intensity was linear, with the concentration of palmatine in the range of 5.0 × 10?9 to 1.0 × 10?6 M. The detection limit was 6.0 × 10?10 M for palmatine. The relative standard deviation for 11 parallel measurements of 1.0 × 10?7 M palmatine was 1.5%. This method was applied to the determination of palmatine in pharmaceutical samples and biological fluids, with satisfactory results. The possible reaction mechanism is discussed briefly.

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Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Suqin Han, Bei Wei, and Fang Hao, "Flow-Injection Post-Chemiluminescence Method for the Determination of Palmatine," Appl. Spectrosc. 67, 747-751 (2013)

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